According to the design scheme of natural resource balance sheet proposed by the author in previous study, this paper takes Zhuxi County, Shiyan City, Hubei Province as a case study and compiles the substance accounting table and value accounting table of Zhuxi County respectively by using statistic methods, market price law, alternative market law, willingness assessment law, etc., and the relationship between the changes of natural resources and regional economic development are analyzed. The results are as follows: 1) The total amount of forest and characteristic resources in Zhuxi County is increasing year by year. The industrial water consumption is decreasing year by year. The efficiency of industrial water is increasing continuously, while the resident living water keeps at a consant level. The water usage for urban public and ecological environment is increasing annually and the trend of agricultural water consumption remains constant. Water consumption is mainly affected by precipitation and other natural conditions. In the future, developing modern agriculture and improving agricultural water efficiency are necessary. In addition, land resources are in the trend of converting to construction land. The government of Zhuxi County should control construction area in a reasonable range. 2) The value of natural resources is in a relatively stable state which shows the natural resources are not over-consumed, and the value of land resources is the highest. The influence of human factors on the value of forest resources is positive and the total value is increasing. The influence of human factors on the value of water resources is negative. 3) With the increase of GDP in Zhuxi County, the total amount of natural resources has not reduced and remains relatively stable. The effect of human factors on the total value of natural resources is positive.