Ecological assessment of sustainable development is one of the superiority fields of international ecological economy and researches on sustainable development.The approach of human appropriation of net primary production(HANPP)can assess the ecological sustainability of human ecological-economic systems through measuring how much the net primary production is appropriated by human beings,which is a newly while important research area.In this paper,we briefly introduced the basic concept,theories of human appropriation of net primary production and its approaches to measure sustainable development.Then to sum up all the latest researches on human appropriation of net primary production,the following four prime issues can be summarized for applying HANPP on ecological assessment of regional sustainable development,that is,the uncertainty of key factors,the threshold of sustainability,the restriction in data collection,and the imports and exports of biological production.