Resources and environment are the key part and base for regional agricultural sustainable development.Ecological safety for agricultural and rural development means that resources and environment on which agricultural and rural development depend are balance,and agro ecosystem is health.There are many characteristics on ecological safety,such as strategy,integrity,regionality,hierarchicity,dynamic and periodicity.As a case study of Jiaxing city,Zhejiang province,the paper discusses the method and process of evaluation of ecological safety on agricultural sustainable development,and identifies an indicator system of ecological safety that has 23 indicators group on resource ecology environment pressure,quality and protection ability.Mathematics model on indicators calculation is set up.Indicator standards on ecological safety are given.The approach to and strategy for constructing ecological safety on agricultural sustainable development are pointed out by taking Jiaxing city as a case in this article as well.