The network of high-speed rail (HSR) adds new elements to the tourism industry in China and produces new changes of tourism destinations. Taking Huangshan City as an example, using tourists flow trajectories and related travel attribute data before and after the opening of Beijing-Fuzhou HSR obtained from field research and network retrieval, the impact of Beijing-Fuzhou HSR on the spatial structure of tourist destination district in Huangshan City was analyzed. The result shows :1）After the opening of HSR, the radius of domestic tourist market extended outwardly, and the tourist flows of Fujian, Shandong, Beijing and other cities along the HSR show the “reverse decay” phenomenon. 2）After the opening of HSR, nodes of tourist destination district changed, and the spatial range tourist destination districts extended. Before and after the opening of HSR, the high-frequency node pairs on the whole network are mainly interregional nodes (such as Huangshang - Yangtze River Delta, Huangshan - Jiangxi Province), supplemented with intro-regional node pairs (such as Huangshan - Hefei, Huangshan - Chizhou, Huangshan - Xuancheng). The function of the whole network is less developed, and there is apparent stratification of the network. After the opening of HSR, the core area of whole network changed. The centralized distribution of core nodes changed to decentralized distribution, so the connection between core areas and marginal areas became loose, and the radiation effect of core area became weaker. The degree centrality values of core nodes and important nodes changed greatly, while the betweens centrality values did not change much. The structural hole levels of core nodes are high, showing the prominent function of the nodes. 3) Before and after the opening of HSR, the spatial structure of the tourist destination districts in Huangshan City has the plate pattern, including Huangshan - Yangtze River Delta plate, southern Anhui tourism area plate (Huangshan - Chizhou - Xuancheng), and Huangshan - Jiangxi plate. The spatial development mode of the destination districts gradually changed from “single core - periphery” to “dual core - periphery”, from cluster to dispersion. The research of the impact of HSR on the tourism development in Huangshan City provides a general reference for the tourism development of Huangshan City and the surrounding areas.