To investigate the silicon (Si) storage in sympodial bamboo ecosystem and its spatial distribution in China, samples were collected from the sympodial bamboo forest in Guangdong, Fujian, Zhejiang, Yunnan, and Sichuang provinces. The eight selected sympodial bamboo species in this study were Bambusa textilis McClure (BTM), Bambusa chungii McClure (BCM), Bambusa burmanica McClure (BBM), Dendrocalamus latiflorus Munro (DLM), Dendrocalamopsis oldhami (Munro) Keng f. (DOK), Dendrocalamus membranceus Munro (DMM), Dendrocalamus giganteus Munro (DGM) and Neosinocalamus affinis (Rendle) Keng f. (NAK), respectively. They cover more than 0.64 million hm2 and approximately account for 80% of the total sympodial bamboo forest area in China. The above-ground Si storage of the eight sympodial bamboo forests was initially estimated by measuring the Si content and dry biomass in leaf, branch, culm and litter, respectively. The results showed that: 1) the Si contents in different organs of the eight sympodial bamboo forests ranked as follows: leaf (12.47-62.71 g·kg-1) > branch (7.66- 29.26 g·kg-1) > culm (1.12-11.77 g·kg-1), and the average Si content in litter was higher than that in organs. Meanwhile, among the different species, the Si content of the leaf and branch was significantly higher in DOK than that in other species. 2) The Si storages of the eight different sympodial bamboo species ranked in the following order: DGM > DMM > NAK > DOK > BTM > BBM > BCM > DLM. In addition, the total Si storage in above-ground part of the eight sympodial bamboo species was about 5082.93 kg·hm-2, in which the Si storage in the litter accounted for more than 51%. And 3) through calculating the area and the Si storage, the storage of total Si in the above-ground part of the eight selected sympodial bamboo species was approximately 41.55×104 t Si and the storage of the total Si in the whole sympodial bamboo in China was approximately 51.94×104 t Si. The initial estimation of Si storage in the whole sympodial bamboo ecosystem in this study has a vital significance and provides basic data for estimating the Si storage in the whole bamboo ecosystem, and even that in subtropical forest ecosystem.