自然资源学报 ›› 2011, Vol. 26 ›› Issue (3): 504-512.doi: 10.11849/zrzyxb.2011.03.017

• 资源研究方法 • 上一篇    下一篇

城镇化过程中地下水微生物的时空分异与演化——以珠海为例

黄小兰1,2, 陈建耀1, 周世宁3, 谢丽纯1, 蒋华波1, 董林垚1   

  1. 1. 中山大学 地理科学与规划学院,广州 510275;
    2. 广西师范学院 资源与环境科学学院,南宁 530001;
    3. 中山大学 生命科学学院,广州 510275
  • 收稿日期:2010-03-15 修回日期:2010-10-22 出版日期:2011-03-20 发布日期:2011-03-20
  • 通讯作者: 陈建耀,教授,博士,博士生导师。E-mail: chenjyao@mail.sysu.edu.cn E-mail:chenjyao@mail.sysu.edu.cn
  • 作者简介:黄小兰(1968- ),女,江西赣州人,副研究员,博士生,主要研究方向为城市化水环境效应。E-mail:huangxiaolanlan@163.com
  • 基金资助:

    广东省自然科学基金项目(9251027501000021);国家自然科学基金项目(40571027);中山大学后备重点课题项目(2008-2009);广东水利科技创新与推广项目(2009年度)。

Spatial Variation of Groundwater Microorganism and Its Implications in Assessing Environmental Evolution Due to Urbanization in Zhuhai, China

HUANG Xiao-lan1,2, CHEN Jian-yao1, ZHOU Shi-ning3, XIE Li-chun1, JIANG Hua-bo1, DONG Lin-yao1   

  1. 1. School of Geography and Planning, Sun Yat-sen University, Guangzhou 510275, China;
    2. School of Resource and Environment Sciences, Guangxi Teachers Education University, Nanning 530001, China;
    3. School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, China
  • Received:2010-03-15 Revised:2010-10-22 Online:2011-03-20 Published:2011-03-20

摘要: 运用不依赖培养的分子生物学技术,选择珠海市唐家湾居民密集地与人类活动扰动较少的中山大学珠海校区共二样区进行多采样点分析,构建16S rRNA基因文库进行序列比对,获得微生物的群落分布数据。数据显示,校区小流域地下水微生物群落基本保持原生态,其上、中、下游三组井均以变形菌、Candidate division OPx、古菌(含泉古菌和广生古菌)为优势类群,其中中游#M组井因环境不同微生物稍有差异。人类活动影响显著的唐家社区其微生物群落结构变异较大,未发现属于微生物鼻祖的古菌,也未检测到Candidate division OPx系列,而检测出许多与周围环境相吻合的微生物群落。二样区地下水微生物16S rRNA基因演化分析表明,城镇化进程中环境变化影响地下水微生物种群发育,地下水微生物也不断演化并影响环境。

关键词: 城镇化, 地下水, 微生物群落, 环境演化

Abstract: Cultured-independent molecular analyzing technology was used to construct 16S rRNA gene library and gene sequences of microbial groups for groundwater samples collected at Zhuhai Campus of Sun Yat-sen University and Tangjiawan Town. Microbiological groups were compared to identify the adaption and possible evolution due to urbanization by using the campus as a background. The Proteobacterium, Candidate division OPx and uncultured archaeon(uncultured Crenarchaeote and Euryarchaeote)were found predominant in the recharge, middle and discharge zones of the campus, while none of the uncultured archaeon and Candidate division OPx were found in Tangjiawan. Although microorganism in the groundwater evolves in response to the change from a background with little anthropogenic influence to a urbanized town, linkages of microbial groups between two sites were detected by the genetic neighbor-joining tree, indicating its evolution under a changed environment.

Key words: urbanization, groundwater, microbial group, environmental evolution

中图分类号: 

  • X143