兴安落叶松林火后对土壤养分和土壤微生物生物量的影响

doi:10.11849/zrzyxb.2011.03.011

摘要/Abstract

摘要： 以大兴安岭兴安落叶松林火后不同强度(重度、中度、轻度)及未火烧区的土壤为研究对象,于火烧结束3年后(2009年)的秋季,采用氯仿熏蒸浸提法测定了不同强度火烧后土壤的微生物生物量碳(C_mic)和微生物生物量氮(N_mic),并研究其与土壤养分因子的关系。结果表明:兴安落叶松林重度火烧区的C_mic显著高于中度、轻度和未火烧区,N_mic在不同强度火烧样地间差异不显著,但在重度火烧区出现最高值。其中重度、中度、轻度和对照的C_mic平均为692.8、499.9、428.8和498.7 mg·kg^-1,而N_mic分别为70.6、55.2、50.9和54.1 mg·kg^-1。土壤含水量、土壤pH值、土壤有机碳对C_mic和N_mic的影响显著,土壤微生物生物量与土壤含水量、pH值、土壤有机碳均呈正相关。研究将为进一步开展火干扰对北方森林土壤碳平衡影响机理研究提供科学依据。

关键词: 林火, 土壤微生物生物量, 氯仿熏蒸浸提法, 兴安落叶松林, 土壤养分

Abstract: For the aim of understanding the relationships between the soil microbial biomass and nutrient content after fire in Larix gmelinii forest in Da Hinggan Mountains of China, detailed field investigations were conducted in burned area with different fire intensities (serious, medium and low fire intensity). To find the impact factors of microbial biomass,we measured soil microbial biomass carbon (C_mic), nitrogen (N_mic) and soil nutrient of different fire intensities with a fumigation-extraction method after burned three years. The results showed that the C_mic in serious burned site was significantly higher than in the medium, low and unburned sites. Though the N_mic has no significant effect in different treatments, yet it was the biggest in serious burned site. Mean soil C_mic in serious burned site was different significantly with medium, low and unburned sites. The mean C_mic of serious, medium, low and unburned sites was 692.8, 499.9, 428.8 and 498.7 mg·kg^-1, and the mean N_mic was 70.6, 55.2, 50.9 and 54.1 mg·kg^-1, respectively. The content of soil organic carbon and total N in serious burned area was 82.6 g·kg^-1 and 4.9 g·kg^-1 respectively, which was higher than in the unburned area significantly and presented an ascending trend with the increase of fire intensity. The content of soil total phosphorus increased significantly, but it has no significant difference among different fire intensities. Soil available phosphorus content increased in light burned area significantly, which was 29.9 mg·kg^-1, but a decrease occurred with the increase of fire intensities. Soil moisture content, pH and soil organic carbon influenced the C_mic and N_mic significantly, the soil microbial biomass was positively correlated to the soil moisture content, pH and soil organic carbon.

Key words: soil microbial biomass, fumigation-extraction method, Larix gmelinii, soil nutrient, forest fire

中图分类号:

S153.6

赵彬, 孙龙, 胡海清, 孙志虎. 兴安落叶松林火后对土壤养分和土壤微生物生物量的影响[J]. 自然资源学报, 2011, 26(3): 450-459.

ZHAO Bin, SUN Long, HU Hai-qing, SUN Zhi-hu. Post-fire Soil Microbial Biomass and Nutrient Content of Larix gmelinii Forest in Autumn[J]. JOURNAL OF NATURAL RESOURCES, 2011, 26(3): 450-459.

参考文献

[1] 李杨, 黄国宏, 史奕. 大气CO₂浓度升高对农田土壤微生物及其相关因素的影响[J]. 应用生态学报, 2003, 14(12): 2321- 2325. [2] Williams M A, Rice C W, Owensby C E. Carbon dynamics and microbial activity in tallgrass prairie exposed to elevated CO₂ for 8 years [J]. Plant and Soil, 2000, 227: 127-137. [3] Hossain A, Raison R J, Khanna P K. Effects of fertilizer application and fire regime on soil microbial biomass carbon and nitrogen, and nitrogen mineralization in an Australian subalpine eucalypt forest [J]. Biology and Fertility of Soils, 1995, 19: 246-252. [4] Knapp A K, Conard S K, Blair J M. Determinants of soil CO₂ flux from a sub-humid grassland: Effects of fire and fire history [J]. Ecological Applications, 1998, 8: 760-770. [5] Wan S, Hui D, Luo Y. Fire effects on nitrogen pool and dynamics in terrestrial ecosystems: A meta-analysis [J]. Ecological Applications, 2001, 11(5): 1349-1365. [6] 罗菊春. 大兴安岭森林火灾对森林生态系统的影响[J]. 北京林业大学学报, 2002, 24(5/6): 101-107. [7] Bastias B A, Xu Z, Cairney J W G. Influence of long-term repeated prescribed burning on mycelial communities of ectomycorrhizal fungi [J]. New Phytologist, 2006, 172: 149-158. [8] Prober S M, Thiele K R, Lunt I D. Fire frequency regulates tussock grass composition, structure and resilience in endangered temperate woodlands [J]. Austral Ecology, 2007, 32: 808-824. [9] Neary D G, Klopatek C C, Debano L F, et al. Fire effects on belowground sustainability: A review and synthesis [J]. Forest Ecology and Management, 1999, 122: 51-71. [10] Hart S C, Deluca T H, Newman G S, et al. Post-fire vegetative dynamics as drivers of microbial community structure and function in forest soils [J]. Forest Ecology and Management, 2005, 220: 166-184. [11] Prieto-Fernandez A, Acea M J, Carballas T. Soil microbial and extractable C and N after wildfire [J]. Biology and Fertility of Soils, 1998, 27: 132-142. [12] Banning N C, Murphy D V. Effect of heat-induced disturbance on microbial biomass and activity in forest soil and the relationship between disturbance effects and microbial community structure [J]. Applied Soil Ecology, 2008, 40: 109-119. [13] Campbell C D, Cameron C M, Bastias B A, et al. Long term repeated burning in a wet sclerophyll forest reduces fungal and bacterial biomass and responses to carbon substrates [J]. Soil Biology ＆ Biochemistry, 2008, 40: 2246-2252. [14] Harden J W, Trumbore S E, Stocks B J, et al. The role of fire in the boreal carbon budget [J]. Global Change Biol., 2000, 6(Supp 1): 174-184. [15] French N H F, Goovaerts P, Kasischke E S. Uncertainty in estimating carbon emissions from boreal forest fires [J]. Journal of Geophysical Research, 2004, 109(D14S08): 1-12. [16] Michelsen A, Andersson M, Jensen M, et al. Carbon stocks, soil respiration and microbial biomass in fire-prone tropical grassland, woodland and forest ecosystems [J]. Soil Biology & Biochemistry, 2004, 36: 1707-1717. [17] 林启美, 吴玉光, 等. 熏蒸法测定土壤微生物量碳的改进[J]. 生态学杂志, 1999, 18(2): 63- 66. [18] Wu J, Joergensen R, Pommerening B, et al. Measurement of soil microbial biomass C by fumigation-extraction—An automated procedure [J]. Soil Biology & Biochemistry, 1990, 22: 1167-1169. [19] Joergensen R, Brookes P C. Ninhydrin-reactive nitrogen measurements of microbial biomass in 0.5 mol K₂SO₄ soil extracts [J]. Soil Biology & Biochemistry, 1990, 22: 1023-1027. [20] Robichaud P R. Fire effects on infiltration rates after prescribed fire in northern Rocky Mountain forests, USA [J]. Journal of Hydrology, 2000(231/232): 220-229. [21] DeBano L F. The role of fire and soil heating on water repellency in wildland environments: A review [J]. Journal of Hydrology, 2000(231/232): 195-206. [22] Ice G G, Neary D G, Adams P W. Effects of wildfire on soils and watershed processes [J]. Journal of Forestry, 2004, 102(6): 16-20. [23] Hamman S T, Burke I C, Stromberger M E. Relationships between microbial community structure and soil environmental conditions in a recently burned system [J]. Soil Biology & Biochemistry, 2007, 39(7): 1703-1711. [24] Almendros G, Martin F, Gonzalez-Vila F J. Effect of fire on humic and lipid fractions in a Dystric Xerochrept in Spain[J]. Geoderma, 1988, 42(2): 115-127. [25] Andriesse J P, Koopmans T T. A monitoring study on nutrient cycles in soils used for shifting cultivation under various climate conditions in tropical Asia I: The influence of simulated burning on form and availability of plant nutrients [J]. Agriculture, Ecosystems & Environment, 1984, 12(1): 1-16. [26] 沙丽清, 邓继武, 谢克金, 等. 西双版纳次生林火烧前后土壤养分变化的研究[J]. 植物生态学报, 1998, 22(6): 513-517. [27] 戴伟. 人工油松林火烧前后土壤化学性质变化的研究[J]. 北京林业大学学报, 1994, 16(1): 102-105. [28] Dyrness C T, Van Cleeve K, Levison J D. The effect of wildfire on soil chemistry in four forest types in interior Alaska [J]. Canadian Journal of Forest Research, 1989, 19(11): 1389-1396. [29] Covington W W, Sackett S S. Soil mineral nitrogen changes following prescribed burning in ponderosa pine [J]. Forest Ecology and Management, 1992, 54: 175-191. [30] Kovacic D A, Swift D M, Ellis J E, et al. Immediate effects of prescribed burning on mineral soil nitrogen in ponderosa pine of New Mexico [J]. Soil Science, 1986, 141: 71-75. [31] Schoch P, Binkley D. Prescribed burning increased nitrogen availability in a mature loblolly pine stand [J]. Forest Ecology and Management, 1986, 14: 13-22. [32] Bell R L, Binkley D. Soil nitrogen mineralization and immobilization in response to periodic prescribed fire in a loblolly pine plantation [J]. Canadian Journal of Forest Research, 1989, 19: 816-820. [33] Raison R J, Khanna P K, Woods P V. Mechanisms of element transfer to the atmosphere during vegetation fires [J]. Canadian Journal of Forest Research, 1985, 15: 132-140. [34] Knoepp J D, Swank W T. Comparison of available soil nitrogen assays in control and burned forest sites [J]. Soil Science Society of America Journal, 1995, 59: 1750-1754. [35] Moghaddas E E Y, Stephens S L. Thinning, burning, and thin-burn fuel treatment effects on soil properties in a Sierra Nevada mixed conifer forests [J]. Forest Ecology and Management, 2007, 250: 156-166. [36] Kutiel P, Naveh Z. The effect of fire on nutrients in a pine forest soil [J]. Plant and Soil, 1987, 104: 269-274. [37] Dumontet S, Dinel H, Scopa A, et al. Post-fire soil microbial biomass and nutrient content of a pine forest soil from a dunal Mediterranean environment [J]. Soil Biology & Biochemistry, 1996, 28(10/11): 1467-1475. [38] Clarholm M. Microbial biomass P, labile P, and acid phosphatase activity in the humus layer of a spruce forest, after repeated additions of fertilizer [J]. Biology and Fertility of Soils, 1993, 8: 128-133. [39] Giai C, Boerner R E J. Effects of ecological restoration on microbial activity, microbial functional diversity, and soil organic matter in mixed-oak forests of southern Ohio, USA [J]. Applied Soil Ecology, 2007, 35: 281-290. [40] Hamman S T, Burke I C, Knapp E E. Soil nutrients and microbial activity after early and late season prescribed burns in a Sierra Nevada mixed conifer forest [J]. Forest Ecology and Management, 2008, 256: 367-374. [41] Andersson M, Michelsen A, Jensen M, et al. Tropical savannah woodland: effects of experimental fire on soil microorganisms and soil emissions of carbon dioxide [J]. Soil Biology & Biochemistry, 2004(36): 849-858. [42] Ilstedt U, Giesler R, Nordgren A, et al. Change in soil chemical and microbial properties after a wildfire in a tropical rainforest in Sabah, Malaysia [J]. Soil Biology & Biochemistry, 2003, 35: 1071-1078. [43] Fierer N, Jackson R B. The diversity and biogeography of soil bacterial communities [J]. Proceedings of the National Academy of Sciences, 2006, 103: 626-631. [44] Bailey V L, Smith J L, Bolton H. Fungal-to-bacterial ratios in soils investigated for enhanced C sequestration [J]. Soil Biology & Biochemistry, 2002, 34: 997-1007. [45] Adams M A, Polglase P J, Attiwill P M, et al. In situ studies of nitrogen mineralization and uptake in forest soils: Some comments on methodology [J]. Soil Biology & Biochemistry, 1989, 21: 423-429. [46] Hook P B, Burke I C. Evaluation of methods for estimating net nitrogen mineralization in a semiarid grassland [J]. Soil Science Society of America Journal, 1995, 59: 831-837. [47] Heisler J L, Briggs J M, Knapp A K, et al. Direct and indirect effects of fire on shrub density and aboveground productivity in a mesic grassland [J]. Ecology, 2004, 85: 2245-2257. [48] Xu W, Wan S. Water-and plant-mediated responses of soil respiration to topography, fire, and nitrogen fertilization in a semi-arid grassland in northern China [J]. Soil Biology & Biochemistry, 2008, 40: 679-687. [49] Arnold S S, Fernandez I J, Rustad L E, et al. Microbial response of an acid forest soil to experimental soil warming [J]. Biology and Fertility of Soils, 1999, 30: 239-244. [50] Chen T H, Chiu C Y, Tian G L. Seasonal dynamics of soil microbial biomass in coastal sand dune forest [J]. Pedobiologia, 2005, 49: 645-653. [51] Schimel J P, Gulledge J M, Clein-Curley J S, et al. Moisture effects on microbial activity and community structure in decomposing birch litter in the Alaskan taiga [J]. Soil Biology & Biochemistry, 1999, 31: 831-838. [52] Zak D R, Pregitzer K R, Curtis P S, et al. Elevated CO₂ and feedback between carbon and nitrogen cycles [J]. Plant and Soil, 1993, 151: 105-107. [53] Zak D R, Tilman D, Parmenter R R, et al. Plant-production and soil-microorganism in late-successional ecosystems—A continental-scale study [J]. Ecology, 1994, 75: 2333-2347. [54] Norby R J, Luo Y. Evaluating ecosystem responses to rising atmospheric CO₂ and global warming in a multi-factor world [J]. New Phytologist, 2004, 162: 281-293.