自然资源学报 ›› 2011, Vol. 26 ›› Issue (3): 440-449.doi: 10.11849/zrzyxb.2011.03.010

• 资源生态 • 上一篇    下一篇

小兴安岭红松外生菌根真菌资源及响应面法优化Suillusgrevillei培养基

宋福强, 刘远开, 杜春梅, 杨峰山   

  1. 黑龙江大学生 命科学学院,哈尔滨 150080
  • 收稿日期:2010-07-30 修回日期:2010-11-08 出版日期:2011-03-20 发布日期:2011-03-20
  • 通讯作者: 杨峰山,主要研究方向为植物保护学。E-mail:yangfshan@126.com E-mail:yangfshan@126.com
  • 作者简介:宋福强(1969- ),男,黑龙江齐齐哈尔市人,教授,博士,主要研究方向为菌根学和微生物生态学。E-mail:0431sfq@163.com
  • 基金资助:

    哈尔滨市科技创新人才研究专项资金项目(2007RFXXN047);国家自然科学基金 (31070576,30571493);黑龙江省大学高层次人才支持计划项目(生态修复团队)。

Diversity of Ectomycorrhizal Macro-fungi at Xiao Hinggan Mountain and Medium Optimization for Suillus grevillei Using Response Surface Methodology

SONG Fu-qiang, LIU Yuan-kai, DU Chun-mei, YANG Feng-shan   

  1. Life Science School of Heilongjiang University, Harbin 150080, China
  • Received:2010-07-30 Revised:2010-11-08 Online:2011-03-20 Published:2011-03-20

摘要: 红松是濒危的珍稀植物,同时又是典型的外生菌根树种。论文对小兴安岭红松林内外生菌根真菌资源进行了调查,同时利用响应面法筛选优化厚环粘盖牛肝菌(Suillus grevillei)培养基的组分。经对小兴安岭红松林下外生菌根真菌采集调查与分类鉴定,初步确定有5个科、8个属、21个种的真菌为红松外生菌根真菌类群,其中常见的有厚环粘盖牛肝菌、灰鹅膏菌(Amanita vaginata)、赭盖红菇(Russula mustelina)等。以改良MMN培养基为基础培养基,利用响应面法优化筛选确定S.grevillei的最佳培养基配方是KH2PO4 0.3 g、Peptone 16 g、Glucose 12 g、Beef extract 15 g、 CaCl2 0.05 g、MgSO4·7H2O 0.15 g、Malt extract 3 g、FeCl3(1%溶液) 1.2 ml、NaCl 0.025 g、(NH4)2HPO4 0.25 g、Vitamin B1微量(0.005 g) 、Agar 20 g并加水至1 000 ml。该配方为S.grevillei菌剂的规模化生产奠定了基础。

关键词: 红松, 外生菌根真菌, 响应面法, 厚环粘盖牛肝菌

Abstract: Pinus koraiensis is a rare and endangered tree species and is also growing typically symbiotic plant dependent on ectomycorrhizal fungi. In this paper, the forest of Pinus koraiensis at Xiao Hinggan Mountain was taken as a destined area for study, and ectomycorrhizal fungal resources and diversity under the forest of P.koraiensis was also investigated, and components of the culturing medium for Suillus grevillei (Kl. ) Sing. was optimized as well, using response surface experiments. First of all, 21 species under 8 genera of 5 families were identified under the Korean pine forest at Xiao Hinggan Mountain, where the ectomycorrhizal macro-fungal resources are rich, with dominating species of Suillus grevillei, Amanita vaginata and Russula mustelina, and the Pinus koraiensis as an ectomycorrhiza host plant. Based on MMN medium, through response surface analysis we confirmed that components of the optimal medium for culturing Suillus grevillei are KH2PO4 0.3 g, peptone 16 g, glucose 12 g, CaCl2 0.05 g, MgSO4·7H2O 0.15 g, malt extract 3.0 g, FeCl3 (1% solution) 1.2 ml, NaCl 0.025 g, (NH4)2HPO4 0.25 g, vitamin B1 trace 0.005 g, agar 20 g, and added water to 1000 ml. The optimized medium would provide basis for large-scale Suillus grevillei production in the future.

Key words: Pinus koraiensis, ectomycorrhizal fungi, response surface methodology, Suillus grevillei

中图分类号: 

  • S791.247